WP 1: Whole-cell modeling of metabolism


1) To develop a whole-cell dynamic model framework of the metabolism of M. pneumoniae

2) To build upon M. pneumoniae models to develop a genome-scale, constraint-based model of M. hyopneumoniae for vaccine optimization

3) To deploy the metabolic model(s) to: a) the rational design and optimization of the vaccine chassis; b) aid the development of a higher-growth rate chassis; c) assist the development of a nutrient optimized a serum-free growth medium and; d) assess, at genome scale, the metabolic capabilities of a series of Mycoplasmas with the purpose of designing a tailored vaccine portfolio